Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: AM22

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	AM22
Locus	AM22-1 AM22-2 AM0022
Reference	Fu YB et al. (2007) Microsatellite variation in Avena sterilis oat germplasm Theoretical and Applied Genetics 114:1029-1038. Li CD et al. (2000) The development of oat microsatellite markers and their use in identifying relationships among Avena species and oat cultivars Theoretical and Applied Genetics 101:1259-1268.
General Remarks	Insert contains repeat (AC)22 Among 20 oat cultivars, 2 alleles were found, polymorphic information content (PIC) = 0.47. In 369 Avena sterilis accessions there were 3 alleles of size 60-146 bp, PIC = 0.34.
Type	Genomic PCR SSR
PCR primers	5'ATT GTA TTT GTA GCCC CCA GTT C 5'AAG AGC GAC CCA GTT GTA TG
SSR size	138
Amplification Conditions	Average melting temperature (degrees C) = 46
Source Species	Avena sativa
Source Germplasm	Ogle (oat)