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GrainGenes Probe Report: AM19

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	AM19
Reference	Fu YB et al. (2007) Microsatellite variation in Avena sterilis oat germplasm Theoretical and Applied Genetics 114:1029-1038. Li CD et al. (2000) The development of oat microsatellite markers and their use in identifying relationships among Avena species and oat cultivars Theoretical and Applied Genetics 101:1259-1268.
General Remarks	Insert contains repeat (AC)3..(AC)6..(AC)5..(AC)7 Among 20 oat cultivars, 1 allele was found. In 369 Avena sterilis accessions there were 4 alleles of size 248-300 bp, PIC = negative.
Type	Genomic PCR SSR
PCR primers	5'ATA GAA CGG CAT GAT AAC GAA ATA 5'GCG CGA CAA CAG GAC CTT C
SSR size	251
Amplification Conditions	Average melting temperature (degrees C) = 48
Source Species	Avena sativa
Source Germplasm	Ogle (oat)