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GrainGenes Probe Report: AM14

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	AM14
Locus	AM0014
Reference	Li CD et al. (2000) The development of oat microsatellite markers and their use in identifying relationships among Avena species and oat cultivars Theoretical and Applied Genetics 101:1259-1268.
General Remarks	Insert contains repeat (AC)21 Known repeat = Mini-Tn5 with 71 % identity over 56 base pairs Among 20 oat cultivars, 6 alleles were found, polymorphic information content (PIC) = 0.69.
Type	PCR SSR
PCR primers	5'GTG GTG GGC ACG GTA TCA 5'TGG GTG GCG AAG CGA ATC
SSR size	133
Amplification Conditions	Average melting temperature (degrees C) = 48
Source Species	Avena sativa
Source Germplasm	Ogle (oat)