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GrainGenes Probe Report: AM4

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	AM4  [ Marker Report ]
Locus	AM4
Reference	Fu YB et al. (2007) Microsatellite variation in Avena sterilis oat germplasm Theoretical and Applied Genetics 114:1029-1038. Li CD et al. (2000) The development of oat microsatellite markers and their use in identifying relationships among Avena species and oat cultivars Theoretical and Applied Genetics 101:1259-1268.
General Remarks	Insert contains repeat (AG)34 Known repeat = 3'-Alu with 70 % identity over 51 base pairs Polymorphic in Kanota/Ogle and Marion/Terra. Among 20 oat cultivars, 5 alleles were found, polymorphic information content (PIC) = 0.68. In 369 Avena sterilis accessions there were 21 alleles of size 114-220 bp, PIC = 0.89.
Type	Genomic PCR SSR Microsatellite
PCR primers	5'GGT AAG GTT TCG AAG AGC AAA G 5'GGG CTA TAT CCA TCC CTC AC
SSR size	166
Amplification Conditions	Average melting temperature (degrees C) = 48
Source Species	Avena sativa
Source Germplasm	Ogle (oat)