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GrainGenes Probe Report: AM2

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	AM2
Reference	Li CD et al. (2000) The development of oat microsatellite markers and their use in identifying relationships among Avena species and oat cultivars Theoretical and Applied Genetics 101:1259-1268.
General Remarks	Insert contains repeat (AG)24 Among 20 oat cultivars, 3 alleles were found, polymorphic information content (PIC) = 0.49.
Type	PCR SSR
PCR primers	5'TGA ATT CGT GGC ATA GTC ACA AGA 5'AAG GAG GGC ATA GGG AGG TAT TT
SSR size	144
Amplification Conditions	Average melting temperature (degrees C) = 49
Source Species	Avena sativa
Source Germplasm	Ogle (oat)