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GrainGenes Probe Report: Bmag853

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	Bmag853  [ Marker Report ]
Locus	Bmag0853 Bmag853
Synonym	See also Bmag0853
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (GA)15 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products Diversity Index (DI) = 0.64
Type	PCR SSR
PCR primers	5' ACAAGTATCCTGCAAACCTAA 3' 5' CGACCTTCTTAATGGTTAGTG 3'
SSR size	183
Amplification Conditions	3 min 94C, 45 cycles of 1 min @94C, 1 min @55C, 2 min @72C, final extension of 10 min @72C. 25 microlitre reactions contained 125 nM of each primer.
Source Species	Hordeum vulgare