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GrainGenes Probe Report: Bmac282

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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
Bmac282  [ Marker Report ]
Locus
Bmac0282a
Bmac0282b
Bmac282
Synonym
Other nameBmac0282
Reference
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (AC)5
Quality = 1: Single product, only weak stutter bands
Type
PCR
SSR
PCR primers
5' CACACATACCACGCATGT 3'
5' ATGTAAAATGGACGTATCACC 3'
SSR size
195
Amplification Conditions
1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare