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GrainGenes Probe Report: Bmac35

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	Bmac35  [ Marker Report ]
Locus	Bmac0035
Synonym	Other name Bmac0035
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AT)10(AC)29 Quality = 5: Bands of unexpected size or additional bands. Very weak bands or no amplification products
Type	PCR SSR
PCR primers	5' TCTCATCATTTTTTTGGGTGG 3' 5' TGTGACCAAATACAAGAGGCC 3'
SSR size	168
Amplification Conditions	'Touchdown' PCR: 18 cycles of denaturing 1 min @94C and extension 1 min @72C, with annealing for 30s with temp decreased 1C every second cycel from 64C to 55C. Continue 30 cyclesfor 1 min @94C, 1 min @55C, 1 min @72C. End with 5 min @72C.
Source Species	Hordeum vulgare