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GrainGenes Marker Report: tin3

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Locus	tin3
Type	Trait
Chromosome	3A
Map	Wheat-Composite2004-3A 61
	[ Show Nearby Loci ]
Species	Triticum aestivum
Probe	CFA2076
Associated Gene	tin3 (Triticum)
Gene Class	Tiller inhibition
Reference	Kuraparthy V et al. (2007) Identification and mapping of a tiller inhibition gene (tin3) in wheat. Theoretical and Applied Genetics 114:265-294.
Remark	Trait marker Xcfa2076-3A Nearby marker on Composite map: Xwmc169 Position on Composite map estimated by GrainGenes.
Probe for Locus	CFA2076
Locus	Xcfa2076-3A [ Show all 6 ]
Reference	Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (TG)18 Regularity of motif is imperfect
Type	PCR SSR
PCR primers	Forward 5' CGAAAAACCATGATCGACAG 3' Reverse 5' ACCTGTCCAGCTAGCCTCCA 3'
SSR size	172
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	3D
Source Species	Triticum urartu