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GrainGenes Marker Report: cfe0143

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Locus	cfe0143
Type	Microsatellite
Map	Barley, B73xCPI-5H 162.3
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Map Data	Barley, B73xCPI
Probe	CFE143
Probe for Locus	CFE143
Locus	cfe0143
Reference	Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. Zhang LY et al. (2005) High transferability of bread wheat EST-derived SSRs to other cereals Theoretical and Applied Genetics 111:677-687.
General Remarks	SSR motif: (CAGG)4
Type	PCR SSR EST
PCR primers	CGACTAACGACCAAAGCACA CATCCACACCCACAAGGAG Left CGACTAACGACCAAAGCACA Right CATCCACACCCACAAGGAG
SSR size	150 theoretical; 166 observed
Amplification Conditions	Annealing temperature 59.9
Sequence	BE426772 CD904199 CD906283 CD906594
Linkage Group	5D
Source Species	Triticum aestivum