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GrainGenes Marker Report: Xwmc522

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Locus	Xwmc522
Type	Microsatellite
Chromosome	2A
Map	Wheat, UC1110 x PI610750 2A 76.9 Wheat, WH542 x Synthetic 2A 77.8 Wheat-Composite2004-2A 55
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Map Data	Wheat, Composite, 2004 Wheat, UC1110 x PI610750 Wheat, WH542 x Synthetic
Probe	WMC522
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xwmc522-2A wmc522-2A wmc522 wmc522-3D
Probe for Locus	WMC522
Locus	Xwmc522-2A Xwmc522 wmc522-2A wmc522 wmc522-3D
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 22h5 Donor: Bright, Zeneca Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 207 bases located on 2A Trimmed to insert at both ends. (CT)36 74 to 145, (TC)4 163 to 170.
Type	PCR SSR
PCR primers	Forward AAAAATcTcAcgAgTcgggc at 5 length 20 bases, Tm 63 Reverse cccgAgcAggAgcTAcAAAT at 178 length 20 bases, Tm 63
SSR size	193 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide (180-200 bases).
Sequence	WMC522
Source Species	Triticum aestivum