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GrainGenes Marker Report: Xwmc47-4B

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Locus	Xwmc47-4B
Type	Microsatellite
Chromosome	4B
Chromosome Arm	4BL
Map	CS/SQ1-4B 132.5 [ Show all 6 ]
	[ Show Nearby Loci ]
Map Data	Durum wheat, Kofa x UC1113 [ Show all 6 ]
Probe	WMC47
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114. Somers DJ et al. (2003) Molecular mapping of novel genes controlling Fusarium head blight resistance and deoxynivalenol accumulation in spring wheat Genome 46:555-564.
Possible Orthologs	Xwmc47 [ Show all 6 ]
Probe for Locus	WMC47
Locus	Xwmc47-4B [ Show all 7 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6f3 Donor: Dubcovsky, Buenos Aires Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 127 bases located on 4B, 144 bases located on 4B AGR70. Trimmed to insert at 3' end. (CA)8 86 to 101. (3 loci).
Type	PCR SSR
PCR primers	Forward gAAAcAgggTTAAccATgccAA at 30 length 22 bases, Tm 65 Reverse ATggTgcTgccAAcAAcATAcA at 149 length 22 bases, Tm 65
SSR size	141 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC47
Source Species	Triticum aestivum