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GrainGenes Marker Report: Xwmc326c

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Locus	Xwmc326c
Type	Microsatellite
Map	Svevo/Ciccio-2A 0
	[ Show Nearby Loci ]
Breakpoint Interval	C-2AS5-0.78
Map Data	Durum wheat, Svevo x Ciccio
Probe	WMC326
Possible Orthologs	Xwmc326 [ Show all 10 ]
Probe for Locus	WMC326
Locus	Xwmc326-3B [ Show all 11 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 17d9 Donor: Khairallah, Mexico Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 188 bases located on 3B Trimmed to insert at 5' end, and to Ssp1 at 3' end. (CT)16 112 to 143.
Type	PCR SSR
PCR primers	Forward ggAgcATcgcAggAcAgA at 22 length 18 bases, Tm 63 Reverse ggAcgAggAcgccTgAAT at 190 length 18 bases, Tm 63
SSR size	186 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide.
Sequence	WMC326
Source Species	Triticum aestivum