GrainGenes Marker Report: Xwmc28-5B
Locus
Xwmc28-5B
Type
Microsatellite
Chromosome
5B
Map
Kofa/UC1113-5B 145.1 Ta-SSR-2004-5B 139.4
[ Show Nearby Loci ]
Map Data
Durum wheat, Kofa x UC1113 Wheat, Consensus SSR, 2004
Probe
WMC28
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc28 wmc28
Probe for Locus
WMC28
Locus
Xwmc28-5B Xwmc28 wmc28
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 2g9 Donor: Wang, Utah One small (CA) and one large (T)(CA) compound.(GT)29 132 to 189
Type
PCR SSR
PCR primers
Forward ATcAcgcATgTcTgcTATgTAT at 42 length 22 bases, Tm 59.0
Reverse ATTAgAccATgAAgAcgTgTAT at 208 length 22 bases, Tm 55.0
SSR size
188 bases expected, Chinese Spring
Amplification Conditions
51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Sequence
WMC28
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc28-5B
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