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GrainGenes Marker Report: Xwmc278-1A

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Locus	Xwmc278-1A
Type	Microsatellite
Chromosome	1A
Map	CS/SQ1-1A 53.3 Louise/Penawawa-1A 61.6 Ta-SSR-2004-1A 61.7
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Map Data	Wheat, Chinese Spring x SQ1 Wheat, Consensus SSR, 2004 Wheat, Louise x Penawawa
Probe	WMC278
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	Xwmc278 wmc278
Probe for Locus	WMC278
Locus	Xwmc278-1A Xwmc278 wmc278
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 3f10 Donor: Keller, Zurich No trimming done to sequence. (GT)14 200 to 227. Bases 200-300 (ie. 3' side of microsatellite) match part of 11b12
Type	PCR SSR
PCR primers	Forward AAAcgATAgTAAAATTAccTcggAT at 117 length 25 bases, Tm 59 Reverse TcAAAAAATAgcAAcTTgAAgAcAT at 257 length 25 bases, Tm 60
SSR size	165 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC278
Source Species	Triticum aestivum