GrainGenes Marker Report: Xwmc274-3B
Locus
Xwmc274-3B
Type
Microsatellite
Chromosome
3B
Map
Ta-SSR-2004-3B 142.2
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004
Probe
WMC274
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
wmc274 wmc274-3B
Probe for Locus
WMC274
Locus
Xwmc274-3B wmc274 wmc274-3B
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 3h8 Donor: Keller, Zurich Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 135 bases located on 3B No trimming done to sequence. (GAAAA)9 microsatellite 145-187.
Type
PCR SSR
PCR primers
Forward AAgcAAgcAgcAAAAcTATcAA at 92 length 22 bases, Tm 61
Reverse gAATgAATgAATgAATcgAggc at 209 length 22 bases, Tm 62
SSR size
139 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product;
Sequence
WMC274
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc274-3B
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