GrainGenes Marker Report: Xwmc25b-2B
Locus
Xwmc25b-2B
Type
Microsatellite
Map
CS/SQ1-2B 35.3
[ Show Nearby Loci ]
Map Data
Wheat, Chinese Spring x SQ1
Probe
WMC25
Possible Orthologs
Xwmc25-2B
[ Show all 18 ]
Probe for Locus
WMC25
Locus
Xwmc25b-2B
[ Show all 19 ]
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 2c9 Donor: Wang, Utah (GT)26 193 to 244. >2 Polymorphic loci.
Type
PCR SSR
PCR primers
Forward TcTggccAggATcAATATTAcT at 102 length 22 bases, Tm 59.0
Reverse TAAgATAcATAgATccAAcAcc at 246 length 22 bases, Tm 53.0
SSR size
166 bases expected, Chinese Spring
Amplification Conditions
51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Sequence
WMC25
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc25b-2B
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