GrainGenes Marker Report: Xwmc213-2B
Locus
Xwmc213-2B
Type
Microsatellite
Chromosome
2B
Chromosome Arm
2BS
Map
Ta-SSR-2004-2B 34.5 Ta-Synthetic/Opata-SSR2-2B 77.9
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004 Wheat, Synthetic x Opata, SSR 2
Probe
WMC213
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc213-1B Xwmc213f wmc213 wmc213-2B wmc213-1B
Probe for Locus
WMC213
Locus
Xwmc213f
[ Show all 6 ]
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 3c6 Donor: Jack, Plant Breeding International No trimming done to insert. (CA)13 36 to 61, (CA)10 88 to 107. Bases 98-195 (ie 3' side of microsatellite) match (weakly) part of 2h12. Reverse primer here is within well matched region.
Type
PCR SSR
PCR primers
Forward ATTTTcTcAAAcAcAccccg at 8 length 20 bases, Tm 62
Reverse TAgcAgATgTTgAcAATggA at 172 length 20 bases, Tm 57
SSR size
184 bases expected, Chinese Spring
Amplification Conditions
51C anneal, product.
Sequence
WMC213
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc213-2B
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