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GrainGenes Marker Report: Xwmc112

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Locus	Xwmc112
Type	Microsatellite
Map	Ta-NxW-2D 36.2 Wheat, UC1110 x PI610750 2D 14.2
	[ Show Nearby Loci ]
Map Data	Wheat, Nanda2419 x Wangshuibai Wheat, UC1110 x PI610750
Probe	WMC112
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xwmc112-2D Xwmc112-3A Xwmc112y wmc112 wmc112-2D
Probe for Locus	WMC112
Locus	Xwmc112-2D [ Show all 6 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 8g8 Donor: Flore, Verneuil Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 251 bases located on 5B AGR170. Trimmed to Rsa1 at both ends. (CA)17 202 to 235, (CT)34 134 to 201. Good clear polymorphism (1 locus).
Type	PCR SSR
PCR primers	Forward TgAgTTgTggggTcTTgTTTgg at 105 length 22 bases, Tm 66.0 Reverse TgAAggAgggcAcATATcgTTg at 328 length 22 bases, Tm 66.0
SSR size	245 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC112
Source Species	Triticum aestivum