GrainGenes Marker Report: Xwmc110-5A
Locus
Xwmc110-5A
Type
Microsatellite
Chromosome
5A
Map
Kofa/UC1113-5A 172.8 Ta-SSR-2004-5A 127.2
[ Show Nearby Loci ]
Map Data
Durum wheat, Kofa x UC1113 Wheat, Consensus SSR, 2004
Probe
WMC110
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
wmc110 wmc110-5A
Probe for Locus
WMC110
Locus
Xwmc110-5A wmc110 wmc110-5A
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 8c8 Donor: Flore, Verneuil Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 172 bases located on 5A AGR166. Reverse sequence only from Lark. Trimmed to insert 5' and Rsa1 at 3'. (GT)11 232 to 253.
Type
PCR SSR
PCR primers
Forward gcAgATgAgTTgAgTTggATTg at 108 length 22 bases, Tm 61.0
Reverse gTAcTTggAAAcTgTgTTTggg at 256 length 22 bases, Tm 60.0
SSR size
170 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence
WMC110
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc110-5A
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