GrainGenes Marker Report: Xwmc0477-2B
Locus
Xwmc0477-2B
Type
Microsatellite
Map
P91193D1/P92201D5-2B 155.2
[ Show Nearby Loci ]
Map Data
Wheat, P91193D1 x P92201D5
Probe
WMC477
Possible Orthologs
Xwmc477-2B wmc477-2B wmc477 Xwmc477
Probe for Locus
WMC477
Locus
Xwmc477-2B Xwmc0477-2B wmc477-2B wmc477 Xwmc477
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 25b4 Donor: Jizeng, Beijing Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 170 bases located on 2B Trimmed to insert at 5' end and to Ssp1 at 3' end. (GT)16 118 to 149.
Type
PCR SSR
PCR primers
Forward cgTcgAAAAccgTAcAcTcTcc at 10 length 22 bases, Tm 65
Reverse gcgAAAcAgAATAgcccTgATg at 155 length 22 bases, Tm 65
SSR size
167 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic on acrylamide.
Sequence
WMC477
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc0477-2B
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