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GrainGenes Marker Report: Xpsp3123-7D

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Locus
Xpsp3123-7D
Type
Microsatellite
Chromosome
7D
Chromosome Arm
7DL
Map
Ta-Courtot/CS-GPW-7D188.7
Ta-Gale-7D24.6
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Map Data
Wheat, Courtot x Chinese Spring, GPW
Wheat, Gale
Probe
PSP3123
Reference
ReferenceStephenson P et al. (1998) Fifty new microsatellite loci for the wheat genetic map. Theoretical and Applied Genetics 97:946-949.
Remark
Quality score = 4 (multiple products and multiple loci, easy to score but sometimes difficult to relate polymrphic loci to those previously characterized and mapped)



Probe for Locus
PSP3123
Locus
Xpsp3123-7D
Xpsp3123
Reference
ReferenceStephenson P et al. (1998) Fifty new microsatellite loci for the wheat genetic map. Theoretical and Applied Genetics 97:946-949.
ReferenceBryan GJ et al. (1997) Isolation and characterisation of microsatellites from hexaploid bread wheat Theoretical and Applied Genetics 94:557-563.
General Remarks
Insert contains repeat (TC)16
Type
PCR
SSR
PCR primers
Forward AGATTAGATGGCATAAGCAAAGAGC
Reverse GATTAATCACCGCCCTTGGTCC
SSR size
178, 7D locus
Amplification Conditions
63C anneal
Sequence
PSP3123
Source Species
Triticum aestivum