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GrainGenes Marker Report: Xpsp3112-4D

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Locus	Xpsp3112-4D
Type	Microsatellite
Chromosome	4D
Chromosome Arm	4DL
Map	Ta-Gale-4D 5.5
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Map Data	Wheat, Gale
Probe	PSP3112
Reference	Stephenson P et al. (1998) Fifty new microsatellite loci for the wheat genetic map. Theoretical and Applied Genetics 97:946-949.
Remark	Quality score = 2 (single product recognizing a single locus, some stuttering however easy to score)
Probe for Locus	PSP3112
Locus	Xpsp3112.1-3B Xpsp3112.2-6B Xpsp3112-4D Xpsp3112.1 Xpsp3112.2
Reference	Stephenson P et al. (1998) Fifty new microsatellite loci for the wheat genetic map. Theoretical and Applied Genetics 97:946-949. Bryan GJ et al. (1997) Isolation and characterisation of microsatellites from hexaploid bread wheat Theoretical and Applied Genetics 94:557-563.
General Remarks	Insert contains repeat (GT)11
Type	PCR SSR
PCR primers	Forward CGAGAGTGTGCGCAGTAGGC Reverse CTTGATACTAATCTATCGACCCGC
SSR size	175, 4D locus
Amplification Conditions	63C anneal
Sequence	PSP3112
Source Species	Triticum aestivum