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GrainGenes Marker Report: Xpsp3103-4D

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Locus	Xpsp3103-4D
Type	Microsatellite
Chromosome	4D
Chromosome Arm	4DS
Map	CS/SQ1-4D 0 Ta-Courtot/CS-GPW-4D 4.4 Ta-Gale-4D -7.5
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Map Data	Wheat, Chinese Spring x SQ1 Wheat, Courtot x Chinese Spring, GPW Wheat, Gale
Probe	PSP3103
Reference	Stephenson P et al. (1998) Fifty new microsatellite loci for the wheat genetic map. Theoretical and Applied Genetics 97:946-949.
Remark	Quality score = 3 (single locus, profound and/or irregular stuttering, sometimes difficult to score)
Probe for Locus	PSP3103
Locus	Xpsp3103-4D Xpsp3103
Reference	Stephenson P et al. (1998) Fifty new microsatellite loci for the wheat genetic map. Theoretical and Applied Genetics 97:946-949. Bryan GJ et al. (1997) Isolation and characterisation of microsatellites from hexaploid bread wheat Theoretical and Applied Genetics 94:557-563.
General Remarks	Insert contains repeat (GA)13 (imperfect)
Type	PCR SSR
PCR primers	Forward CGGCTGGCATATTGGAATGTGC Reverse GCTTCCGTGCTGTGGTTGGTC
SSR size	162, 4D locus
Amplification Conditions	61C anneal
Sequence	PSP3103
Source Species	Triticum aestivum