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GrainGenes Marker Report: Xpsp3100-1B

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Locus	Xpsp3100-1B
Type	Microsatellite
Chromosome	1B
Chromosome Arm	1BL
Map	Ta-Courtot/CS-GPW-1B 136.4 Ta-Gale-1B 60.2
	[ Show Nearby Loci ]
Map Data	Wheat, Courtot x Chinese Spring, GPW Wheat, Gale
Probe	PSP3100
Reference	Stephenson P et al. (1998) Fifty new microsatellite loci for the wheat genetic map. Theoretical and Applied Genetics 97:946-949.
Remark	Quality score = 2 (single product recognizing a single locus, some stuttering however easy to score)
Probe for Locus	PSP3100
Locus	Xpsp3100-1B Xpsp3100
Reference	Stephenson P et al. (1998) Fifty new microsatellite loci for the wheat genetic map. Theoretical and Applied Genetics 97:946-949. Bryan GJ et al. (1997) Isolation and characterisation of microsatellites from hexaploid bread wheat Theoretical and Applied Genetics 94:557-563.
General Remarks	Insert contains repeat (GA)20
Type	PCR SSR
PCR primers	Forward TAGGTCTAAGGGTAAATGTAGGC Reverse ACTCTCGCCGGTGGTGGCCTC
SSR size	167, 1B locus
Amplification Conditions	61C anneal
Sequence	PSP3100
Source Species	Triticum aestivum