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GrainGenes Marker Report: Xpsp3078-3B

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Locus
Xpsp3078-3B
Type
Microsatellite
Chromosome
3B
Chromosome Arm
3BL
Map
Ta-Gale-3B72.5
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Map Data
Wheat, Gale
Probe
PSP3078
Reference
ReferenceStephenson P et al. (1998) Fifty new microsatellite loci for the wheat genetic map. Theoretical and Applied Genetics 97:946-949.
Remark
Quality score = 4 (multiple products and multiple loci, easy to score but sometimes difficult to relate polymrphic loci to those previously characterized and mapped)



Probe for Locus
PSP3078
Locus
Xpsp3078-4B
Xpsp3078-3B
Xpsp3078
Reference
ReferenceStephenson P et al. (1998) Fifty new microsatellite loci for the wheat genetic map. Theoretical and Applied Genetics 97:946-949.
ReferenceBryan GJ et al. (1997) Isolation and characterisation of microsatellites from hexaploid bread wheat Theoretical and Applied Genetics 94:557-563.
General Remarks
Insert contains repeat (TG)11
Type
PCR
SSR
PCR primers
Forward TTTGCAGCTTGACATATAGGTAG
Reverse AAAAGCGGCAAGATGCACCTTG
SSR size
178, 3B locus
Amplification Conditions
61C anneal
Sequence
PSP3078
Source Species
Triticum aestivum