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GrainGenes Marker Report: Xmwg897-6B

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Locus	Xmwg897-6B
Type	RFLP
Map	Tturgidum-Du-6B 47
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Map Data	T. turgidum, durum x dicoccoides
Probe	MWG897
Possible Orthologs	MWG897 Xmwg897 Xmwg897-6A
Probe for Locus	MWG897
Locus	MWG897 Xmwg897-6B Xmwg897-6A Xmwg897
Reference	Marcel TC et al. (2007) A high-density consensus map of barley to compare the distribution of QTLs for partial resistance to Puccinia hordei and of defence gene homologues. Theoretical and Applied Genetics 114:487-500. Sayed-Tabatabaei BE et al. (1998) DNA sequencing and primer designing for RFLP clones evenly distributed in the barley genome. Barley Genetics Newsletter 28:15-18.
General Remarks	Restriction Enzyme for CAPS marker is Dde I
Type	Genomic STS
PCR primers	5' ggatcagtgatttcgccgtgtttct 3' 5' ccatgatgcaaagctccaactactc 3'
STS primers	T7 side (5' to 3') GGATCAGTGATTTCGCCGTGTTTCT T3 side (5' to 3') CCATGATGCAAAGCTCCAACTACTC
Amplification Conditions	5 min at 94 deg C; 35 cycles with 30 sec at 94 deg C, 30 sec at 63 deg C, and 1 min at 72 deg C; and a final extension step of 7 min at 72 deg C
Sequence	AJ234632 AJ234631 MWG897 MWG897-5 MWG897-3
Linkage Group	6H
Insert Enzyme	PstI
Source Species	Hordeum vulgare
Insert Size	1.2