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GrainGenes Marker Report: Xgwm456-1B

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Locus	Xgwm456-1B
Type	Microsatellite
Map	Ta-Grandin/BR34-1B 32
	[ Show Nearby Loci ]
Map Data	Wheat, Grandin x BR34
Probe	WMS456
Possible Orthologs	Xgwm456 Xgwm456-3D
Probe for Locus	WMS456
Locus	Xgwm456-3D Xgwm456-1B Xgwm456 S-D1
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GA)21 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' TCTGAACATTACACAACCCTGA 3' 5' TGCTCTCTCTGAACCTGAAGC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS456
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring