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GrainGenes Marker Report: Xgwm349b

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Locus	Xgwm349b
Type	Microsatellite
Chromosome	2D
Map	Wheat-Composite2004-2D 117
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004
Probe	WMS349
Possible Orthologs	Xgwm349-2D Xgwm349a Xgwm349 gwm349
Probe for Locus	WMS349
Locus	Xgwm349-2D Xgwm349a Xgwm349b gwm349 Xgwm349
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GA)34 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' GGCTTCCAGAAAACAACAGG 3' 5' ATCGGTGCGTACCATCCTAC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS349
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring