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GrainGenes Marker Report: Xgwm332

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Locus	Xgwm332
Type	Microsatellite
Chromosome	7A
Chromosome Arm	7AL
Map	Svevo/Ciccio-7A 128.2 Tt-LxG-7A 104.2 Wheat, UC1110 x PI610750 7A 156
	[ Show Nearby Loci ]
Breakpoint Interval	7AL21-0.74-0.86
Map Data	Durum wheat, Svevo x Ciccio T. turgidum, Langdon x G18-16 Wheat, UC1110 x PI610750
Probe	WMS332
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xgwm332-7A [ Show all 7 ]
Probe for Locus	WMS332
Locus	Xgwm332-7A [ Show all 8 ]
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GA)36 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' AGCCAGCAAGTCACCAAAAC 3' 5' AGTGCTGGAAAGAGTAGTGAAGC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS332
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring