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GrainGenes Marker Report: Xgwm320

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Locus	Xgwm320
Type	Microsatellite
Chromosome	2D
Map	Wheat, Avalon x Cadenza 2D2 10.1 Wheat-Composite2004-2D 113
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Map Data	Wheat, Avalon x Cadenza Wheat, Composite, 2004
Probe	WMS320
Possible Orthologs	Xgwm320-2D Xgwm320-2R gwm320
Probe for Locus	WMS320
Locus	Xgwm320-2D Xgwm320 Xgwm320-2R gwm320
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GT)9(GA)15 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' CGAGATACTATGGAAGGTGAGG 3' 5' ATCTTTGCAAGGATTGCCC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS320
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring