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GrainGenes Marker Report: Xgwm319-7A

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Locus
Xgwm319-7A
Type
Microsatellite
Map
USG3209/Jaypee-7A122
[ Show Nearby Loci ]
Map Data
Wheat, USG3209 x Jaypee
Probe
WMS319
Possible Orthologs
Xgwm319
Xgwm319-2B
gwm319
gwm319-2B



Probe for Locus
WMS319
Locus
Xgwm319-2B
[ Show all 6 ]
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (CT)11(N)23(CT)6
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR
SSR
PCR primers
5' GGTTGCTGTACAAGTGTTCACG 3'
5' CGGGTGCTGTGTGTAATGAC 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS319
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring