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GrainGenes Marker Report: Xgwm111.2-2B

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Locus
Xgwm111.2-2B
Type
Microsatellite
Map
CS/SQ1-2B223.4
[ Show Nearby Loci ]
Map Data
Wheat, Chinese Spring x SQ1
Probe
WMS111
Possible Orthologs
Xgwm111
[ Show all 17 ]



Probe for Locus
WMS111
Locus
Xgwm111.2-2B
[ Show all 19 ]
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (CT)32(GT)17
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR
SSR
PCR primers
5' TCTGTAGGCTCTCTCCGACTG 3'
5' ACCTGATCAGATCCCACTCG 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS111
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring