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GrainGenes Marker Report: Xcfd9-3D

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Locus
Xcfd9-3D
Type
Microsatellite
Chromosome
3D
Chromosome Arm
3DL
Map
Chinese_Spring_Deletion_SSR_3D0.905
Louise/Penawawa-3D124.3
Ta-Courtot/CS-GPW-3D227.1
Ta-SSR-2004-3D35.7
Triticum-AxF-3D204.8
[ Show Nearby Loci ]
QTL with Locus Marker
Qsst.msub-3DL
Breakpoint Interval
3DL3-0.81-1.00
Map Data
Wheat, Arina x Forno
Wheat, Consensus SSR, 2004
Wheat, Courtot x Chinese Spring, GPW
Wheat, Louise x Penawawa
Wheat, Physical, SSR
Species
Triticum aestivum
Probe
CFD9
Reference
ReferenceSomers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
ReferencePaillard S et al. (2003) An integrative genetic linkage map of winter wheat (Triticum aestivum L.) Theoretical and Applied Genetics 107:1235-1242.
Possible Orthologs
Xcfd9
cfd9
cfd9-3D.2



Probe for Locus
CFD9
Locus
Xcfd9-3D
Xcfd9
cfd9
cfd9-3D.2
Reference
ReferenceGuyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks
Quality Score = A
Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems)
Amplifies motif: (TC)29
Regularity of motif is perfect
Type
PCR
SSR
PCR primers
Forward 5' TTGCACGCACCTAAACTCTG 3'
Reverse 5' CAAGTGTGAGCGTCGG 3'
SSR size
209
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
3D
Source Species
Aegilops tauschii