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GrainGenes Marker Report: Xcfd88

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Locus	Xcfd88
Type	Microsatellite
Chromosome	4A
Map	Wheat, WH542 x Synthetic 4A 106.8 Wheat-Composite2004-4A 108
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Map Data	Wheat, Composite, 2004 Wheat, WH542 x Synthetic
Probe	CFD88
Possible Orthologs	Xcfd88-4A cfd88
Probe for Locus	CFD88
Locus	Xcfd88-4A Xcfd88 cfd88
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = C Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CCG)5 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' TAGGCATAGTTTTGGGCCTG 3' Reverse 5' GGTAGAAGGAAGCTTCGGGA 3'
SSR size	238
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	4A
Source Species	Aegilops tauschii