GrainGenes Marker Report: Xcfd84-4D
Locus
Xcfd84-4D
Type
Microsatellite
Chromosome
4D
Chromosome Arm
4DL
Map
Chinese_Spring_Deletion_SSR_4D 0.155 Ta-Courtot/CS-GPW-4D 84.2 Ta-SSR-2004-4D 66.7 Triticum-AxF-4D 10
[ Show Nearby Loci ]
Breakpoint Interval
C-4DL9-0.31
Map Data
Wheat, Arina x Forno Wheat, Consensus SSR, 2004 Wheat, Courtot x Chinese Spring, GPW Wheat, Physical, SSR
Species
Triticum aestivum
Probe
CFD84
Reference
Possible Orthologs
Xcfd84 cfd84
Probe for Locus
CFD84
Locus
Xcfd84-4D Xcfd84 cfd84
Reference
Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks
Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GA)26 Regularity of motif is perfect
Type
PCR SSR
PCR primers
Forward 5' GTTGCCTCGGTGTCGTTTAT 3'
Reverse 5' TCCTCGAGGTCCAAAACATC 3'
SSR size
193
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
4D
Source Species
Aegilops tauschii
GrainGenes Marker Report: Xcfd84-4D
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