GrainGenes Marker Report: Xcfd82-6A
Locus
Xcfd82-6A
Type
Microsatellite
Map
Chinese_Spring_Deletion_SSR_6A -0.5 P91193D1/P92201D5-6A 0 Ta-Courtot/CS-GPW-6A 117.2 Wheat, Xiaoyan 54 x Jing 411_6A 26.1
[ Show Nearby Loci ]
Breakpoint Interval
6AS1-0.35-0.65
Map Data
Wheat, Courtot x Chinese Spring, GPW Wheat, P91193D1 x P92201D5 Wheat, Physical, SSR Wheat, Xiaoyan 54 x Jing 411_6A
Probe
CFD82
Possible Orthologs
Xcfd82
Probe for Locus
CFD82
Locus
Xcfd82-6A Xcfd82
Reference
General Remarks
Quality Score = C Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (TGC)5 Regularity of motif is perfect
Type
PCR SSR
PCR primers
Forward 5' GCTGATGCTGCTGTAAGTGC 3'
Reverse 5' TGAAGAATACAATGGCAGCAA 3'
SSR size
242
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
6A
Source Species
Aegilops tauschii
GrainGenes Marker Report: Xcfd82-6A
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