GrainGenes Marker Report: Xcfd81-5D
Locus
Xcfd81-5D
Type
Microsatellite
Chromosome
5D
Chromosome Arm
5DS
Map
CS/SQ1-5D 51.9 Louise/Penawawa-5D 0 Ta-Courtot/CS-GPW-5D 38.6 Ta-SSR-2004-5D 28 Triticum-AxF-5D 21
[ Show Nearby Loci ]
Map Data
Wheat, Arina x Forno Wheat, Chinese Spring x SQ1 Wheat, Consensus SSR, 2004 Wheat, Courtot x Chinese Spring, GPW Wheat, Louise x Penawawa
Species
Triticum aestivum
Probe
CFD81
Reference
Possible Orthologs
Xcfd81-4D
[ Show all 6 ]
Probe for Locus
CFD81
Locus
Xcfd81-5D
[ Show all 7 ]
Reference
Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks
Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GT)14(GA)19 Regularity of motif is perfect
Type
PCR SSR
PCR primers
Forward 5' TATCCCCAATCCCCTCTTTC 3'
Reverse 5' GTCAATTGTGGCTTGTCCCT 3'
SSR size
283
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
7B/5D/4D
Source Species
Aegilops tauschii
GrainGenes Marker Report: Xcfd81-5D
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