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GrainGenes Marker Report: Xcfd80-6D

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Locus
Xcfd80-6D
Type
Microsatellite
Chromosome
6D
Map
CS/SQ1-6D93.4
Louise/Penawawa-6D103.8
Ta-Courtot/CS-GPW-6D25.7
Ta-SSR-2004-6D59.5
[ Show Nearby Loci ]
Map Data
Wheat, Chinese Spring x SQ1
Wheat, Consensus SSR, 2004
Wheat, Courtot x Chinese Spring, GPW
Wheat, Louise x Penawawa
Probe
CFD80
Reference
ReferenceSomers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xcfd80
Xcfd80-6A
cfd80
cfd80-6A
cfd80-6D



Probe for Locus
CFD80
Locus
Xcfd80-6D
[ Show all 6 ]
Reference
ReferenceGuyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks
Quality Score = B
Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems)
Amplifies motif: (GA)27
Regularity of motif is perfect
Type
PCR
SSR
PCR primers
Forward 5' ATAGGGGTTTTGAATCACTCC 3'
Reverse 5' TTGGATTTGCAGAGCCTTCT 3'
SSR size
179
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
6D
Source Species
Aegilops tauschii