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GrainGenes Marker Report: Xcfd80

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Locus	Xcfd80
Type	Microsatellite
Chromosome	6D
Map	Ta-NxW-6A 70.1 Wheat, Xiaoyan 54 x Jing 411_6A 3.3 Wheat-Composite2004-6D 76
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai Wheat, Xiaoyan 54 x Jing 411_6A
2 Point Data	TaGW2-6A/Xcfd80 Distance: 0.6 cM
Probe	CFD80
Possible Orthologs	Xcfd80-6A Xcfd80-6D cfd80 cfd80-6A cfd80-6D
Probe for Locus	CFD80
Locus	Xcfd80-6D [ Show all 6 ]
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GA)27 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' ATAGGGGTTTTGAATCACTCC 3' Reverse 5' TTGGATTTGCAGAGCCTTCT 3'
SSR size	179
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	6D
Source Species	Aegilops tauschii