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GrainGenes Marker Report: Xcfd78

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Locus	Xcfd78
Type	Microsatellite
Chromosome	5D
Map	Ta-NxW-5D 52.4 Wheat-Composite2004-5D 15
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Map Data	Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai
Probe	CFD78
Possible Orthologs	Xcfd78-5D cfd78
Probe for Locus	CFD78
Locus	Xcfd78-5D Xcfd78 cfd78
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GA)19 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' ATGAAATCCTTGCCCTCAGA 3' Reverse 5' TGAGATCATCGCCAATCAGA 3'
SSR size	182
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	5D
Source Species	Aegilops tauschii