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GrainGenes Marker Report: Xcfd77-2D

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Locus	Xcfd77-2D
Type	Microsatellite
Map	Chinese_Spring_Deletion_SSR_2D -0.165 Ta-Courtot/CS-GPW-2D 70.3
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Breakpoint Interval	C-2DS1-0.33
Map Data	Wheat, Courtot x Chinese Spring, GPW Wheat, Physical, SSR
Probe	CFD77
Possible Orthologs	Xcfd77a
Probe for Locus	CFD77
Locus	Xcfd77-2D Xcfd77a
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = C Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GGC)6 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' CTGCTTCAGGGATTGGAGAG 3' Reverse 5' GTTTCCTGGGCTAAACCACA 3'
SSR size	209
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	2D
Source Species	Aegilops tauschii