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GrainGenes Marker Report: Xcfd76

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Locus	Xcfd76
Type	Microsatellite
Chromosome	6D
Map	Ta-NxW-6D 141 Wheat-Composite2004-6D 89
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Map Data	Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai
Probe	CFD76
Possible Orthologs	Xcfd76-6D cfd76-6D cfd76
Probe for Locus	CFD76
Locus	Xcfd76-6D Xcfd76 cfd76-6D cfd76
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GA)26 Regularity of motif is imperfect
Type	PCR SSR
PCR primers	Forward 5' GCAATTTCACACGCGACTTA 3' Reverse 5' CGCTCGACAACATGACACTT 3'
SSR size	166
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	6D
Source Species	Aegilops tauschii