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GrainGenes Marker Report: Xcfd75-6D

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Locus	Xcfd75-6D
Type	Microsatellite
Chromosome	6D
Map	Louise/Penawawa-6D 28.9 Ta-Courtot/CS-GPW-6D -27.2 Ta-SSR-2004-6D 19.1
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Map Data	Wheat, Consensus SSR, 2004 Wheat, Courtot x Chinese Spring, GPW Wheat, Louise x Penawawa
Probe	CFD75
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	Xcfd75 cfd75-6D cfd75
Probe for Locus	CFD75
Locus	Xcfd75-6D Xcfd75 cfd75-6D cfd75
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (TGGA)3(GA)23 Regularity of motif is imperfect
Type	PCR SSR
PCR primers	Forward 5' GCATAAACTTGGACCCTGGA 3' Reverse 5' GCTAAGCCACGCTACCACTC 3'
SSR size	297
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	6D
Source Species	Aegilops tauschii