GrainGenes Marker Report: Xcfd73-2B
Locus
Xcfd73-2B
Type
Microsatellite
Chromosome
2B
Map
Chinese_Spring_Deletion_SSR_2B 0.18 Ta-SSR-2004-2B 82.4 Ta-Synthetic/Opata-GPW-2B 83 Wheat, UC1110 x PI610750 2B 97.3
[ Show Nearby Loci ]
Breakpoint Interval
C-2BL2-0.36
Map Data
Wheat, Consensus SSR, 2004 Wheat, Physical, SSR Wheat, Synthetic x Opata, GPW Wheat, UC1110 x PI610750
Probe
CFD73
Reference
Possible Orthologs
Xcfd73
[ Show all 7 ]
Probe for Locus
CFD73
Locus
Xcfd73-2D
[ Show all 8 ]
Reference
Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks
Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CT)19 Regularity of motif is perfect
Type
PCR SSR
PCR primers
Forward 5' GATAGATCAATGTGGGCCGT 3'
Reverse 5' AACTGTTCTGCCATCTGAGC 3'
SSR size
242
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
2B/2D
Source Species
Aegilops tauschii
GrainGenes Marker Report: Xcfd73-2B
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