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GrainGenes Marker Report: Xcfd73

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Locus	Xcfd73
Type	Microsatellite
Chromosome	2B
Map	Wheat-Composite2004-2B 67
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004
Probe	CFD73
Possible Orthologs	Xcfd73-2B [ Show all 7 ]
Probe for Locus	CFD73
Locus	Xcfd73-2D [ Show all 8 ]
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CT)19 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' GATAGATCAATGTGGGCCGT 3' Reverse 5' AACTGTTCTGCCATCTGAGC 3'
SSR size	242
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	2B/2D
Source Species	Aegilops tauschii