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GrainGenes Marker Report: Xcfd70-3D

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Locus	Xcfd70-3D
Type	Microsatellite
Map	Chinese_Spring_Deletion_SSR_3D -0.775 P91193D1/P92201D5-3D 30.9 Ta-Courtot/CS-GPW-3D 102.4
	[ Show Nearby Loci ]
Breakpoint Interval	3DS6-0.55-1.00
Map Data	Wheat, Courtot x Chinese Spring, GPW Wheat, P91193D1 x P92201D5 Wheat, Physical, SSR
Probe	CFD70
Possible Orthologs	Xcfd70 [ Show all 6 ]
Probe for Locus	CFD70
Locus	Xcfd70-3D [ Show all 6 ]
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = D Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CA)19 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' GTCGGCATAGTCGCACATAC 3' Reverse 5' ACTATGCCAAGGGGAGTGTG 3'
SSR size	172
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	3D
Source Species	Aegilops tauschii