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GrainGenes Marker Report: Xcfd69-7D

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Locus	Xcfd69-7D
Type	Microsatellite
Chromosome	7D
Map	Chinese_Spring_Deletion_SSR_7D 0.91 Ta-Courtot/CS-GPW-7D 269.4 Ta-SSR-2004-7D 144.8
	[ Show Nearby Loci ]
QTL with Locus Marker	QPro.inra-7D
Breakpoint Interval	7DL3-0.82-1.00
Map Data	Wheat, Consensus SSR, 2004 Wheat, Courtot x Chinese Spring, GPW Wheat, Physical, SSR
Probe	CFD69
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	Xcfd69
Probe for Locus	CFD69
Locus	Xcfd69-7D Xcfd69
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CA)46 Regularity of motif is imperfect
Type	PCR SSR
PCR primers	Forward 5' AAATACCTTGAATTGTGAGCTGC 3' Reverse 5' TCTGTTCATCCCCAAAGTCC 3'
SSR size	240
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	7D
Source Species	Aegilops tauschii