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GrainGenes Marker Report: Xcfd65b

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Locus	Xcfd65b
Type	Microsatellite
Chromosome	1B
Map	Wheat-Composite2004-1B 27
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004
Probe	CFD65
Possible Orthologs	Xcfd65-1A [ Show all 10 ]
Probe for Locus	CFD65
Locus	Xcfd65b-1B [ Show all 11 ]
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CT)32 Regularity of motif is imperfect
Type	PCR SSR
PCR primers	Forward 5' AGACGATGAGAAGGAAGCCA 3' Reverse 5' CCTCCCTTGTTTTTGGGATT 3'
SSR size	199
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	1D/1B
Source Species	Aegilops tauschii