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GrainGenes Marker Report: Xcfd63-1D

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Locus	Xcfd63-1D
Type	Microsatellite
Chromosome	1D
Chromosome Arm	1DL
Map	Ta-Courtot/CS-GPW-1D 119.2 Ta-SSR-2004-1D 84.1 Triticum-AxF-1D 104.5
	[ Show Nearby Loci ]
Map Data	Wheat, Arina x Forno Wheat, Consensus SSR, 2004 Wheat, Courtot x Chinese Spring, GPW
Species	Triticum aestivum
Probe	CFD63
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114. Paillard S et al. (2003) An integrative genetic linkage map of winter wheat (Triticum aestivum L.) Theoretical and Applied Genetics 107:1235-1242.
Possible Orthologs	Xcfd63 cfd63
Probe for Locus	CFD63
Locus	Xcfd63-1D Xcfd63 cfd63
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = A Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CA)26 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' TCCTGAGGATGTTGAGGACC 3' Reverse 5' GAGAGAGGCGAAACATGGAC 3'
SSR size	275
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	1D
Source Species	Aegilops tauschii